Products / LC-MS/MS ClinMass® Complete Kits
25-OH Vitamin D2 / D3
The ClinMass® Complete Kits with order nos. MS7000, MS7100 and MS7200 enable a fast on-line SPE LC-MS/MS analysis of 25-OH-Vitamin D (D2, D3) with an injection interval of 3 min only.
The C-3 epimer of 25-OH vitamin D3 (3-epi-25-OH vitamin D3) is separated reliably from the analyte peak. An analytical column with a run time of 4 min (order no. MS7035) is additionally available in order to obtain baseline separation.
Sample Pretreatment

Chromatography

Ordering Information
| Order No. | Description | Quantity |
|---|---|---|
| MS7000 | ClinMass® Complete Kit for 25-OH-Vitamin D2/D3 | 1 pce. |
| in Plasma and Serum | ||
| for 300 assays | ||
| Contents: | ||
| Autosampler Washing Solution | 1 x MS7005 | |
| SPE-Buffer | 2 x MS7009 | |
| Mobile Phase | 1 x MS7010 | |
| Internal Standard IS | 1 x MS7012 | |
| Serum Calibrator Set, lyophil. (Level 0 – 3) | 1 x MS7013 | |
| Sample Pretreatment Vials | 3 x MS7020 | |
| Precipitant P | 1 x MS7021 | |
| Separately available components: | ||
| MS7005 | Autosampler Washing Solution | 1000 ml |
| MS7009 | SPE-Buffer | 1000 ml |
| MS7010 | Mobile Phase | 700 ml |
| MS7012 | Internal Standard IS | 30 ml |
| MS7013 | Serum Calibrator Set, lyophil. (Level 0 – 3) | 4 x 1 x 1 ml |
| MS7014 | Optimisation Mix | 2 ml |
| MS7020 | Sample Pretreatment Vials | 100 pcs. |
| MS7021 | Precipitant P | 15 ml |
| Start Accessories: | ||
| MS7030 | Analytical Column with test chromatogram | 1 pce. |
| MS7031 | SPE-Column | 1 pce. |
| Accessories: | ||
| FK7400 | Inline-Filter (stainless steel sieve, free of dead volume) | 1 pce. |
| FK7340 | Sealings and sieves for order no. FK7400 | 4 pcs. each |
| ClinChek® Controls: | ||
| MS7080 | Serum Control, lyophil., Level I | 10 x 1 ml |
| MS7081 | Serum Control, lyophil., Level II | 10 x 1 ml |
| MS7082 | Serum Control, lyophil., Level I, II | 2 x 5 x 1 ml |
In order to meet laboratory demands in high sample throughput for Vitamin D RECIPE has developed a fully automated sample preparation kit for many liquid handling devices with the option to use 96-well-filter-plates as well. The C-3 epimer of 25-OH vitamin D3 (3-epi-25-OH vitamin D3) is separated reliably from the analyte peak. An analytical column with a run time of 4 min (order no. MS7035) is additionally available in order to obtain baseline separation.
Sample Processing

Ordering Information
| Order No. | Description | Quantity |
|---|---|---|
| MS7100 | ClinMass® Complete Kit for 25-OH-Vitamin D2/D3 | 1 pce. |
| in Plasma and Serum | ||
| for 1200 assays | ||
| Contents: | ||
| Autosampler Washing Solution | 4 x MS7005 | |
| SPE-Buffer | 8 x MS7009 | |
| Mobile Phase | 4 x MS7010 | |
| Internal Standard IS | 4 x MS7012 | |
| Serum Calibrator Set, lyophil. (Level 0 – 3) | 4 x MS7013 | |
| 96-Well-Plates (600 µl) | 3 x FK6513 | |
| Precipitant P | 4 x MS7021 | |
| Separately available components: | ||
| MS7005 | Autosampler Washing Solution | 1000 ml |
| MS7009 | SPE-Buffer | 1000 ml |
| MS7010 | Mobile Phase | 700 ml |
| MS7012 | Internal Standard IS | 30 ml |
| MS7013 | Serum Calibrator Set, lyophil. (Level 0 – 3) | 4 x 1 x 1 ml |
| MS7014 | Optimisation Mix | 2 ml |
| FK6513 | 96-Well-Plates (600 µl) | 5 pcs. |
| MS7021 | Precipitant P | 15 ml |
| Start Accessories: | ||
| MS7030 | Analytical Column with test chromatogram | 1 pce. |
| MS7031 | SPE-Column | 1 pce. |
| Accessories: | ||
| FK6501 | Protective Sheets for 96-Well-Plates (PE/PP foil, 80 x 140 mm) | 50 pcs. |
| FK6514 | 96-Well-Plates (300 µl) | 5 pcs. |
| FK7400 | Inline-Filter (stainless steel sieve, free of dead volume) | 1 pce. |
| FK7340 | Sealings and sieves for order no. FK7400 | 4 pcs. each |
| ClinChek® Controls: | ||
| MS7080 | Serum Control, lyophil., Level I | 10 x 1 ml |
| MS7081 | Serum Control, lyophil., Level II | 10 x 1 ml |
| MS7082 | Serum Control, lyophil., Level I, II | 2 x 5 x 1 ml |
In order to meet laboratory demands in high sample throughput for Vitamin D RECIPE has developed a fully automated sample preparation kit for many liquid handling devices with the option to use 96-well-filter-plates as well. The C-3 epimer of 25-OH vitamin D3 (3-epi-25-OH vitamin D3) is separated reliably from the analyte peak. An analytical column with a run time of 4 min (order no. MS7035) is additionally available in order to obtain baseline separation.
Sample Processing

Ordering Information
| Order No. | Description | Quantity |
|---|---|---|
| MS7200 | ClinMass® Complete Kit for 25-OH-Vitamin D2/D3 | 1 pce. |
| in Plasma and Serum with Filter-Plates | ||
| for 1200 assays | ||
| Contents: | ||
| Autosampler Washing Solution | 4 x MS7005 | |
| SPE-Buffer | 8 x MS7009 | |
| Mobile Phase | 4 x MS7010 | |
| Internal Standard IS | 4 x MS7012 | |
| Serum Calibrator Set, lyophil. (Level 0 – 3) | 4 x MS7013 | |
| 96-Well-Plates (300 µl) | 3 x FK6514 | |
| 96-Well-Filter-Plates (1000 µl) | 3 x FK6521 | |
| Precipitant P | 4 x MS7021 | |
| Separately available components: | ||
| MS7005 | Autosampler Washing Solution | 1000 ml |
| MS7009 | SPE-Buffer | 1000 ml |
| MS7010 | Mobile Phase | 700 ml |
| MS7012 | Internal Standard IS | 30 ml |
| MS7013 | Serum Calibrator Set, lyophil. (Level 0 – 3) | 4 x 1 x 1 ml |
| MS7014 | Optimisation Mix | 2 ml |
| FK6514 | 96-Well-Plates (300 µl) | 5 pcs. |
| FK6521 | 96-Well-Filter-Plates (1000 µl) | 5 pcs. |
| MS7021 | Precipitant P | 15 ml |
| Start Accessories: | ||
| MS7030 | Analytical Column with test chromatogram | 1 pce. |
| MS7031 | SPE-Column | 1 pce. |
| Accessories: | ||
| FK6501 | Protective Sheets for 96-Well-Plates (PE/PP foil, 80 x 140 mm) | 50 pcs. |
| FK7400 | Inline-Filter (stainless steel sieve, free of dead volume) | 1 pce. |
| FK7340 | Sealings and sieves for order no. FK7400 | 4 pcs. each |
| ClinChek® Controls: | ||
| MS7080 | Serum Control, lyophil., Level I | 10 x 1 ml |
| MS7081 | Serum Control, lyophil., Level II | 10 x 1 ml |
| MS7082 | Serum Control, lyophil., Level I, II | 2 x 5 x 1 ml |
Download product brochures, product documents (e.g. safety data sheets), and instructions for use.