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25-OH Vitamin D2 / D3 by HPLC

The determination of 25-OH vitamin D2/ D3 is used for the assessment of the vitamin D status.

The RECIPE HPLC complete kit enables a quick and reliable quantification of both analytes from plasma and serum. The sample preparation consists of a simple precipitation step, a time-consuming solid phase extraction is not required. The subsequent HPLC analysis is performed with an injection interval of 10 min only. For system check, calibration and internal quality control a ClinTest® Test Solution, a ClinCal® Serum Calibrator (traceable to NIST standard SRM 972a) and ClinChek® Serum Controls are available.

Sample Preparation


Sample Preparation 25-OH Vitamin D2 D3 Plasma Serum by HPLC

Chromatography


Chromatogram 25-OH Vitamin D2 D3 Plasma Serum by HPLC

Ordering Information


Order No.


Description


Quantity


35000


ClinRep® Complete Kit for 25-OH Vitamin D2 / D3 in Plasma and Serum for 100 assays


1 pce.


Kit Components (order no. 35000):


35010


Mobile Phase


1000 ml


35012


Internal Standard IS


50 ml


35013


Serum Calibrator, lyophil.


5 x 2 ml


35014


Test Solution, lyophil.


2 x 2 ml


35020


Sample Preparation Vials with Reagent, lyophil.


100 pcs.


35021


Precipitant P


50 ml


Manual


1 pce.


Quick Reference


1 pce.


Start Accessories:


35030


Analytical Column with test chromatogram


1 pce.


35032


Guard Column Holder incl. 1 Guard Column


1 pce.


Accessories:


35033


Guard Column


5 pcs.


FK5810


PEEK tubing (connection of prefilter and column)


1 pce.


FK5820


Stainless steel prefilter holder


1 pce.


FK5821


Replacement filter for stainless steel prefilter holder


10 pcs.


ClinChek® Controls:


35080


Serum Control, lyophil., Level I


10 x 2 ml


35081


Serum Control, lyophil., Level II


10 x 2 ml


35082


Serum Control, lyophil., Level I, II


2 x 5 x 2 ml



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