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Catecholamines

With this complete kit the determination of norepinephrine, epinephrine and dopamine is performed from urine.

The sample preparation is simplified for laboratory routine. For a safe and convenient check-up of the pH-value prior to sample extraction, a special stabilising reagent with colour indicator is available. The correctly coloured indicator assures the setting of the appropriate pH value range (pH 2 – 5). Alternatively to this method with manual sample preparation, RECIPE offers ClinRep® Complete Kits for automatic pipetting systems from Gilson.

Sample Preparation


Sample Preparation Catecholamines in Urine

Chromatography


Chromatogram Catecholamines in Urine

Ordering Information


Order No.


Description


Quantity


2000


ClinRep® Complete Kit for Catecholamines in Urine for 100 assays


1 pce.


Kit Components (order no. 2000):


1210


Mobile Phase


1000 ml


2011


Standard Solution


10 ml


2012


Internal Standard IS


3 ml


2013


Urine Calibrator, lyophil.


5 x 8 ml


2020


Sample Preparation Columns


2 x 2020 (2 x 50 pcs.)


2021


Stabilising Reagent S


500 ml


2024


Eluting Reagent E


2 x 2024 (2 x 300 ml)


Manual


1 pce.


Quick Reference


1 pce.


Start Accessories:


2030


Analytical Column with test chromatogram


1 pce.


Accessories:


FK4000


Column Rack (for 20 columns)


1 pce.


ClinChek® Controls:


8820


Urine Control, lyophil., Level I


10 x 8 ml


8821


Urine Control, lyophil., Level II


10 x 8 ml


8822


Urine Control, lyophil., Level I, II


2 x 5 x 8 ml



Catecholamines are important parameters for the diagnosis of pheochromocytomas. In addition to the ClinRep® Complete Kit with manual sample preparation (order no. 2000) RECIPE offers ClinRep® Complete Kits for automated sample preparation with robotic systems from Gilson. Kits for 100 and 500 assays are available.

Sample Preparation*


Sample Preparation Catecholamines Urine Gilson

* The indicated sample/reagent volumes are exemplified for the ASPEC™ XL. The corresponding data for the HT400E are available on request.

Ordering Information


Order No.


Description


Quantity


2200


ClinRep® Complete Kit for Catecholamines in Urine with Gilson ASPEC™ XL for 100 assays


1 pce.


Kit components (order no. 2200):


1210


Mobile Phase


1000 ml


2011


Standard Solution


10 ml


2012


Internal Standard IS


3 ml


2013


Urine Calibrator, lyophil.


5 x 8 ml


2220


Sample Preparation Columns


2 x 2220 (2 x 50 pcs.)


2021


Stabilising Reagent S


500 ml


2224


Eluting Reagent E


400 ml


Manual


Quick Reference


2300


ClinRep® Complete Kit for Catecholamines in Urine with Gilson ASPEC™ XL for 500 assays


1 pce.


Kit components (order no. 2300):


5-fold amount of kit components with order no. 2200 (1210, 2011, …, 2224)


5 x 2200


2400


ClinRep® Complete Kit for Catecholamines in Urine with HTA, Model HT400E for 100 assays


1 pce.


Kit components (order no. 2400):


1210


Mobile Phase


1000 ml


2011


Standard Solution


10 ml


2012


Internal Standard IS


2 x 2012 (2 x 3 ml)


2013


Urine Calibrator, lyophil.


5 x 8 ml


2420


Sample Preparation Columns


2 x 2420 (2 x 50 pcs.)


2421


Stabilising Reagent S


250 ml


2424


Eluting Reagent E


250 ml


FK6401


Sample vials, clear glass, 4ml


2 x FK6401 (2 x 100 pcs.)


Manual


1 pce.


Quick Reference


1 pce.


2500


ClinRep® Complete Kit for Catecholamines in Urine with HTA, Model HT400E for 500 assays


1 pce.


Kit components (order no. 2500):


5-fold amount of kit components with order no. 2400 (1210, 2011, …, FK6401)


5 x 2400


Components for 2200, 2300, 2400, 2500


Start Accessories:


2030


Analytical Column with test chromatogram


1 pce.


ClinChek® Controls:


8820


Urine Control, lyophil., Level I


10 x 8 ml


8821


Urine Control, lyophil., Level II


10 x 8 ml


8822


Urine Control, lyophil., Level I, II


2 x 5 x 8 ml



This ClinRep® Complete Kit is designed for the convenient on-line SPE HPLC analysis of the catecholamines. A time-consuming manual sample preparation is not required with this method. Prior to the on-line analysis the samples sim- ply have to be spiked with the internal standard (one step sample pretreatment).

Sample Pretreatment (Liquid Handling System)


Sample Preparation Catecholamines Urine on-line

Chromatography


Sample Pretreatment Catecholamines Urine on-line

Ordering Information


Order No.DescriptionQuantity
2100ClinRep® Complete Kit for Catecholamines in Urine1 pce.
for 500 assays
Contents:
SPE-Buffer4 x 2109
Mobile Phase6 x 2110
Standard Solution1 x 2011
Urine Calibrator, lyophil.1 x 2013
Manual
Quick Reference
Separately available components:
2109SPE-Buffer2500 ml
2110Mobile Phase2500 ml
2011Standard Solution10 ml
2012Internal Standard IS3 ml
2013Urine Calibrator, lyophil.5 x 8 ml
Start Accessories:
1232Guard Column Holder1 pce.
1233Guard Columns4 pcs.
2130Analytical Column with test chromatogram1 pce.
2131SPE-Column1 pce.
FK5810PEEK capillary connector (connection of prefilter and column)1 pce.
FK5820Stainless steel prefilter holder incl. 1 prefilter1 pce.
FK7400Inline-Filter (stainless steel sieve, free of dead volume)1 pce.
Accessories:
FK1102Switching valve Sykam (6-port / 3-channel incl. electr. drive, PEEK)1 pce.
FK5821Replacement filter for Stainless steel prefilter holder10 pcs.
FK7340Sealings and sieves for order no. FK74004 pcs. each
ClinChek® Controls:
8820Urine Control, lyophil.10 x 8 ml
Level I
8821Urine Control, lyophil.10 x 8 ml
Level II
8822Urine Control, lyophil.2 x 5 x 8 ml
Level I, II

Download product brochures, product documents (e.g. safety data sheets), and instructions for use.

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